Saturday, 4 February 2012
Does Illumina also have a problem with DNA homopolymers?
Apparently it does, according to a note published on the Ion Community website (free registration required). While it is widely known that both Roche 454 and Ion Torrent are in troubles when counting the number of bases in DNA homopolymer tracts, MiSeq seems to have difficulty getting the base after the run correct, often missing the correct call for one more call of the homopolymer. I'm wondering if HiSeq and GAIIx are also affected by the problem. For more information have a look at this post on the Omics! Omics! blog.